KMID : 0368420080510030202
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Journal of Plant Biology 2008 Volume.51 No. 3 p.202 ~ p.202
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Molecular characterization of newS-RNases (¡®S31¡¯ and ¡®S32¡¯) in apple (Malus x domestica Borkh.)¡¯) in apple (Malus x domestica Borkh.)
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Kim Hoy-Taek
Park Jong-In Hirata Yutaka Nou Ill-Sup
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Abstract
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Apple exhibits gametophytic self-incompatibility (GSI) that is controlled by the multiallelic S-locus. This S-locus encodes polymorphicS ribonuclease (S-RNase) for the pistil-part 5 determinant. Information aboutS-genotypes is important when selecting pollen donors for fruit production and breeding of new cultivars. We determined the 5-genotypes of ¡®Charden¡¯ (S2S3S4), ¡®Winesap¡¯ (S1S28), ¡®York Imperial¡¯ (S2S31), ¡®Stark Earliblaze¡¯ (S1S28), and ¡®Burgundy¡¯ (S20S32), byS-RNase sequencing and S-allele-specific PCR analysis. Two newS-RNases, S31 and S32, were also identified from ¡®York Imperial¡¯ and ¡®Burgundy¡¯, respectively. These newS-alleles contained the conserved eight cysteine residues and two histidine residues essential for RNase activity. Whereas S31 showed high similarity to S20 (94%), S32 exhibited 58% (to S24) to 76% (to S25) similarity in the exon regions. We designed newS-allele-specific primers for amplifying S31- and S32-RNasc-specific fragments; these can serve as specific gene markers. We also rearranged the apple S-allele numbers containing those newS-RNases. They should be useful, along with anS-RNase-based PCR system, in determining S-genotypes and analyzing new alleles from apple cultivars.
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KEYWORD
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Apple, gametophytic self-incompatibility, Malus domestica, S-genotype, S-RNase
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